Researchers have made a significant breakthrough in the fabrication of synthetic spider silk.
The new work paves the way for a new era of sustainable clothing production.
Scientists have long been intrigued by the remarkable properties of spider silk, which is stronger than steel yet incredibly lightweight and flexible.
Since engineering recombinant spider silk in 2018 using bacteria, Fuzhong Zhang, a professor of energy, environmental, and chemical engineering at the McKelvey School of Engineering at Washington University in St. Louis, has been working to increase the yield of silk threads produced from microbes while maintaining its desirable properties of enhanced strength and toughness.
Higher yields will be critical if synthetic silk is to be used in everyday applications, particularly in the fashion industry where renewable materials are much in demand to stem the environmental impacts that come from producing an estimated 100 billion garments and 92 million tons of waste each year.
With the help of an engineered mussel foot protein, Zhang created new spider silk fusion proteins, called bi-terminal Mfp fused silks (btMSilks). Microbial production of btMSilks have eightfold higher yields than recombinant silk proteins, and the btMSilk fibers have substantially improved strength and toughness while being lightweight.
As reported in Nature Communications, this could revolutionize clothing manufacturing by providing a more eco-friendly alternative to traditional textiles.
“The outstanding mechanical properties of natural spider silk come from its very large and repetitive protein sequence,” Zhang says. “However, it is extremely challenging to ask fast-growing bacteria to produce a lot of repetitive proteins.
“To solve this problem, we needed a different strategy,” he says. “We went looking for disordered proteins that can be genetically fused to silk fragments to promote molecular interaction, so that strong fibers can be made without using large repetitive proteins. And we actually found them right here in work we’ve already been doing on mussel foot proteins.”
Mussels secrete these specialized proteins on their feet to stick to things. Zhang and his collaborators engineered bacteria to produce them and engineer them as adhesives for biomedical applications. As it turns out, mussel foot proteins are also cohesive, which enables them to stick to each other well, too. By placing mussel foot protein fragments at the ends of his synthetic silk protein sequences, Zhang created a less repetitive, lightweight material that’s at least twice as strong as recombinant spider silk.
The yields on Zhang’s material increased eightfold compared with past studies, reaching 8 grams of fiber material from 1 liter of bacterial culture. This output constitutes enough fabric to test for use in real products.
“The beauty of synthetic biology is that we have lots of space to explore,” Zhang says. “We can cut and paste sequences from various natural proteins and test these designs in the lab for new properties and functions. This makes synthetic biology materials much more versatile than traditional petroleum-based materials.”
In coming work, Zhang and his team will expand the tunable properties of their synthetic silk fibers to meet the exact needs of each specialized market.
“Because our synthetic silk is made from cheap feedstock using engineered bacteria, it presents a renewable and biodegradable replacement for petroleum-derived fiber materials like nylon and polyester,” Zhang says.
The US Department of Agriculture and the National Science Foundation supported the work.
Author Profile
- Futurity is a nonprofit website that aggregates news articles about scientific research conducted at prominent universities in the United States, the United Kingdom, Canada, Europe, Asia, and Australia. It is hosted and edited by the University of Rochester.
Latest entries
- ScienceOctober 30, 2024People don’t like stories they think AI wrote
- ScienceOctober 29, 20244 tips for safer trick-or-treating this Halloween
- ScienceOctober 28, 2024Humans’ love for carbs may date back to before farming
- ScienceOctober 27, 2024Higher quality ‘good cholesterol’ particles tied to better memory